Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody

CASP3 encodes caspase 3 which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Caspase 3 cleaves and activates caspases 6, 7 and 9, and caspase 3 itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of CASP3 results in two transcript variants that encode the same protein.

The Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody is a highly specific and sensitive tool designed for the detection and quantification of cleaved caspase-3 p17 protein in various biological samples. This antibody is produced using a polyclonal approach, ensuring a high affinity and specificity towards the target protein.

Caspase-3 is a crucial enzyme involved in the execution phase of apoptosis, playing a pivotal role in cell death regulation. Cleaved caspase-3 p17 is the active fragment of caspase-3, generated upon proteolytic cleavage during apoptosis induction. Therefore, the detection and quantification of cleaved caspase-3 p17 can serve as a reliable marker for apoptotic cell death.

The Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody exhibits exceptional performance in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been extensively validated for its specificity and sensitivity, ensuring accurate and reproducible results.

This antibody is supplied as a liquid formulation, allowing for easy handling and convenient storage. It is provided in a ready-to-use format, eliminating the need for additional reagents or preparations. The Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody is compatible with a wide range of sample types, including cell lysates, tissue extracts, and cultured cells.

In summary, the Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody is a reliable and efficient tool for the detection and quantification of cleaved caspase-3 p17 protein. Its high specificity, sensitivity, and versatility make it an ideal choice for researchers and scientists studying apoptosis and cell death pathways.