Cdc27 Polyclonal Antibody

The protein encoded by CDC27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and CDC27 product of Schizosaccharomyces pombe nuc 2. Cell division cycle 27 is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.

The Cdc27 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Cdc27 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Cdc27 protein. The resulting polyclonal antibody has been purified using protein A affinity chromatography and is suitable for use in a variety of applications, including Western blotting, immunoprecipitation, and immunohistochemistry.

The Cdc27 protein is a key component of the anaphase-promoting complex/cyclosome (APC/C), which is a large multi-subunit E3 ubiquitin ligase that plays a critical role in the regulation of cell cycle progression and mitosis. The Cdc27 protein is essential for the proper function of the APC/C, and its expression and activity are tightly regulated during the cell cycle.

The Cdc27 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in detecting Cdc27 protein in various cell lines and tissues. It has been shown to recognize both the full-length and truncated forms of Cdc27 protein, and it does not cross-react with other APC/C subunits or related proteins.

Overall, the Cdc27 Polyclonal Antibody is a reliable and high-quality tool for the detection and analysis of Cdc27 protein in biological samples. Its specificity and sensitivity make it an ideal choice for researchers studying the regulation of cell cycle progression and mitosis, as well as those investigating the role of the APC/C in various cellular processes.