Caspase-9 Polyclonal Antibody

CASP9 (caspase 9) encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.

The Caspase-9 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Caspase-9 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Caspase-9 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Caspase-9 Polyclonal Antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It has been validated for use in human, mouse, and rat samples and has been shown to exhibit minimal cross-reactivity with other caspase family members.

This antibody is an essential tool for researchers studying apoptosis and cell death pathways. It enables the detection and quantification of Caspase-9 protein levels in various biological samples, providing valuable insights into the mechanisms of cell death and disease progression. The Caspase-9 Polyclonal Antibody is a reliable and high-quality reagent that is backed by rigorous quality control testing and a satisfaction guarantee.