Calpain 2 Polyclonal Antibody

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. CAPN2 (calpain 2) encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for CAPN2.

The Calpain 2 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of Calpain 2 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human Calpain 2 protein. The antibody has been purified using protein A affinity chromatography and is supplied as a liquid formulation in phosphate-buffered saline (PBS) with 0.02% sodium azide as a preservative.

The Calpain 2 Polyclonal Antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence. It recognizes a single band of approximately 80 kDa in Western blot analysis of human, mouse, and rat tissue lysates. The antibody has also been shown to be highly specific for Calpain 2 protein, with no cross-reactivity with other Calpain family members.

This antibody is an essential tool for researchers studying the role of Calpain 2 in various biological processes, including cell signaling, apoptosis, and cytoskeletal remodeling. Its high specificity and sensitivity make it an ideal choice for detecting Calpain 2 protein in complex biological samples. The Calpain 2 Polyclonal Antibody is available in various sizes to meet the needs of different research applications.