BRCA1 Polyclonal Antibody

BRCA1encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein (BRCA1, DNA repair associated) combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

The BRCA1 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of BRCA1 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human BRCA1 protein. The resulting polyclonal antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

The BRCA1 Polyclonal Antibody is a valuable tool for researchers studying the role of BRCA1 in various cellular processes, including DNA repair, cell cycle regulation, and tumor suppression. This antibody has been shown to detect endogenous levels of BRCA1 protein in various human cell lines and tissues, making it an ideal choice for studying the expression and localization of BRCA1 in normal and diseased tissues.

In addition to its high specificity and sensitivity, the BRCA1 Polyclonal Antibody is also highly versatile and can be used in a variety of applications. This antibody has been successfully used in Western blotting to detect BRCA1 protein in cell lysates and tissue extracts, as well as in immunohistochemistry and immunofluorescence to visualize the localization of BRCA1 in cells and tissues.

Overall, the BRCA1 Polyclonal Antibody is a reliable and effective tool for researchers studying the role of BRCA1 in various biological processes. Its high specificity, sensitivity, and versatility make it an ideal choice for a wide range of applications, from basic research to clinical diagnostics.