BRAF35 Polyclonal Antibody

The breast cancer susceptibility gene (BRCA1) localizes to chromosome 17q. Mutations within this gene account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast cancer, but unlike BRCA1, BRCA2 does not confer a substantially elevated risk of ovarian cancer. The BRCA2-Associated Factor 35 (BRAF35) protein forms a complex with BRCA2, which associates with condensed chromatin during histone H3 phosphorylation. BRAF35 expression levels are highest in proliferating tissues and parallel BRCA2 expression patterns. The structure of BRAF35 includes a kinesin-like coiled coil domain and a nonspecific DNA binding HMG domain. The chromatin localization of BRAF35 and antibody microinjection studies indicate a role for the BRAF35/BRCA2 complex in cell cycle regulation.

The BRAF35 Polyclonal Antibody is a highly specific and sensitive antibody designed for the detection of BRAF35 protein in various biological samples. This antibody is produced by immunizing rabbits with a synthetic peptide corresponding to the C-terminal region of human BRAF35 protein. The antibody has been extensively validated for its specificity and sensitivity in various applications, including Western blotting, immunohistochemistry, and immunofluorescence.

The BRAF35 Polyclonal Antibody is a valuable tool for researchers studying the role of BRAF35 protein in various cellular processes, including transcriptional regulation, chromatin remodeling, and DNA repair. This antibody can be used to detect endogenous levels of BRAF35 protein in various cell lines and tissues, as well as to monitor changes in protein expression levels under different experimental conditions.

The BRAF35 Polyclonal Antibody is supplied as a purified IgG fraction, which has been affinity-purified using a protein A column. The antibody is provided in a liquid format, which can be easily diluted in various buffers for use in different applications. The antibody is also available in different sizes and formats to meet the specific needs of different research applications.

Overall, the BRAF35 Polyclonal Antibody is a reliable and high-quality antibody that can be used for the detection of BRAF35 protein in various biological samples. Its specificity, sensitivity, and versatility make it an ideal tool for researchers studying the role of BRAF35 protein in various cellular processes.