Feiyuebio
SKU:FY-EH3530
Human AFM(Afamin) ELISA Kit
Human AFM(Afamin) ELISA Kit
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Product Name
Human PDE4D(Phosphodiesterase 4D, cAMP Specific) ELISA Kit
Alias
PDE4D ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDE4D ELISA Kit allows for the in vitro quantitative determination of PDE4D concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDE4D and the recovery rates were calculated by comparing the measured value to the expected amount of PDE4D in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
96 |
|
EDTA plasma(n=5) |
86-105 |
97 |
|
heparin plasma(n=5) |
90-98 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE4D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-97% |
93-102% |
86-105% |
|
EDTA plasma(n=5) |
82-99% |
88-97% |
83-101% |
|
heparin plasma(n=5) |
82-92% |
80-92% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
