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ELK Biotechnology

ELK9349

Human sCD130(Soluble CD130) ELISA Kit

Human sCD130(Soluble CD130) ELISA Kit

$458.00 USD
$458.00 USD
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Human sCD130(Soluble CD130) ELISA Kit

Overview

Product name: Human sCD130(Soluble CD130) ELISA Kit
Reactivity: Human
Alternative Names: CDw130; CD130; IL6ST; GP130-RAPS; IR6RB; IL6R-Beta; IR6RB; IR6-RB; Interleukin 6 Signal Transducer; Oncostatin M Receptor; Interleukin-6 Receptor Subunit Beta
Assay Type: Sandwich
Sensitivity: 48 pg/mL
Standard: 8000 pg/mL
Detection Range: 125-8000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sCD130. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sCD130. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sCD130, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sCD130 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
8000.00 2.045 1.977
4000.00 1.628 1.560
2000.00 1.128 1.060
1000.00 0.827 0.759
500.00 0.487 0.419
250.00 0.369 0.301
125.00 0.258 0.190
0.00 0.068 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sCD130 and the recovery rates were calculated by comparing the measured value to the expected amount of sCD130 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 79-97% 88%
Heparin plasma(n=5) 96-108% 102%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sCD130 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-96% 82-93% 82-96% 92-103%
EDTA plasma(n=5) 90-99% 89-102% 87-102% 85-94%
Heparin plasma(n=5) 87-101% 95-102% 89-98% 95-103%
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