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ELK Biotechnology
SKU:ELK9341
Fish HSP70(Heat Shock Protein 70) ELISA Kit
Fish HSP70(Heat Shock Protein 70) ELISA Kit
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$588.00 USD
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Fish HSP70(Heat Shock Protein 70) ELISA Kit
Overview
Product name: | Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit |
Reactivity: | Zebrafish |
Alternative Names: | DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70 |
Assay Type: | Sandwich |
Sensitivity: | 1.27 ng/mL |
Standard: | 200 ng/mL |
Detection Range: | 3.13-200 ng/mL |
Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Assay Length: | 3.5h |
Research Area: | Tumor immunity;Infection immunity;Developmental science; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
200.00 | 1.946 | 1.865 |
100.00 | 1.686 | 1.605 |
50.00 | 1.157 | 1.076 |
25.00 | 0.813 | 0.732 |
12.50 | 0.617 | 0.536 |
6.25 | 0.326 | 0.245 |
3.13 | 0.226 | 0.145 |
0.00 | 0.081 | 0.000 |

Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant HSP-70/HSPA9 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70/HSPA9 in samples.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 90-105% | 97% |
EDTA plasma(n=5) | 87-95% | 91% |
Heparin plasma(n=5) | 82-95% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70/HSPA9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 81-95% | 98-105% | 85-96% | 82-92% |
EDTA plasma(n=5) | 86-94% | 81-95% | 85-97% | 87-96% |
Heparin plasma(n=5) | 80-94% | 90-99% | 87-103% | 83-96% |