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ELK Biotechnology

ELK9330

Human CD177(CD177 antigen) ELISA Kit

Human CD177(CD177 antigen) ELISA Kit

$458.00 USD
$458.00 USD
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Human CD177(CD177 antigen) ELISA Kit

Overview

Product name: Human CD177(CD177 antigen) ELISA Kit
Reactivity: Human
Alternative Names: CD177; NB1; PRV1; CD177 antigen; Human neutrophil alloantigen 2a; HNA-2a; NB1 glycoprotein; NB1 GP; Polycythemia rubra vera protein 1; PRV-1; CD177
Assay Type: Sandwich
Sensitivity: 29 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD177. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD177. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD177, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD177 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 2.096 1.994
2500.00 1.593 1.491
1250.00 1.186 1.084
625.00 0.871 0.769
312.50 0.527 0.425
156.25 0.262 0.160
78.13 0.256 0.154
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant CD177 and the recovery rates were calculated by comparing the measured value to the expected amount of CD177 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 81-97% 89%
Heparin plasma(n=5) 83-99% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD177 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 93-105% 88-101% 86-97%
EDTA plasma(n=5) 87-102% 89-101% 89-105% 90-101%
Heparin plasma(n=5) 87-93% 95-106% 88-103% 81-98%
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