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ELK Biotechnology

ELK9314

Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit

Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit

$376.00 USD
$376.00 USD
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Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit

Overview

Product name: Human PGF2a(Prostaglandin F2 Alpha) ELISA Kit
Reactivity: Human
Alternative Names: PGF2A; PGF2-A; PG-F2a
Assay Type: Competitive Inhibition
Sensitivity: 3.88 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2a in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 0.198 —
400.00 0.386 —
200.00 0.692 —
100.00 0.856 —
50.00 1.169 —
25.00 1.592 —
12.50 1.869 —
0.00 2.226 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PGF2a and the recovery rates were calculated by comparing the measured value to the expected amount of PGF2a in samples.
Matrix Recovery range Average
serum(n=5) 78-96% 87%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGF2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 87-96% 85-93% 82-90%
EDTA plasma(n=5) 93-102% 93-106% 87-101% 91-104%
Heparin plasma(n=5) 87-98% 92-100% 88-104% 87-93%
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