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ELK Biotechnology

ELK9253

Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

$458.00 USD
$458.00 USD
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Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Overview

Product name: Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit
Reactivity: Human
Alternative Names: ACA-IgG; Anti-Cardiolipin Antibody IgG
Assay Type: Sandwich
Sensitivity: 1.032 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 1.929 1.843
100.00 1.543 1.457
50.00 1.115 1.029
25.00 0.821 0.735
12.50 0.557 0.471
6.25 0.319 0.233
3.13 0.206 0.120
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACA-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgG in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 87-101% 94%
Heparin plasma(n=5) 89-103% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 79-96% 92-105% 91-99%
EDTA plasma(n=5) 90-98% 87-98% 92-104% 86-98%
Heparin plasma(n=5) 81-98% 78-90% 91-99% 97-103%
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