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ELK Biotechnology

ELK9238

Human anti-CarP(anti-carbamylated protein) ELISA Kit

Human anti-CarP(anti-carbamylated protein) ELISA Kit

$458.00 USD
$458.00 USD
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Human anti-CarP(anti-carbamylated protein) ELISA Kit

Overview

Product name: Human anti-CarP(anti-carbamylated protein) ELISA Kit
Reactivity: Human
Alternative Names: anti- CarP
Assay Type: Sandwich
Sensitivity: 0.32 ng/mL
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Biochemical research
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human anti-CarP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human anti-CarP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human anti-CarP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human anti-CarP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
50.00 2.201 2.119
25.00 1.612 1.530
12.50 1.193 1.111
6.25 0.875 0.793
3.13 0.557 0.475
1.57 0.357 0.275
0.79 0.219 0.137
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant anti-CarP and the recovery rates were calculated by comparing the measured value to the expected amount of anti-CarP in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 80-93% 86%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of anti-CarP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 82-96% 95-102% 87-1028%
EDTA plasma(n=5) 85-97% 96-105% 95-103% 91-104%
Heparin plasma(n=5) 82-94% 85-96% 78-90% 85-98%
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