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ELK Biotechnology

ELK9217

Human NKX2-2(NK2 Homeobox Protein 2) ELISA Kit

Human NKX2-2(NK2 Homeobox Protein 2) ELISA Kit

$458.00 USD
$458.00 USD
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Human NKX2-2(NK2 Homeobox Protein 2) ELISA Kit

Overview

Product name: Human NKX2-2(NK2 Homeobox Protein 2) ELISA Kit
Reactivity: Human
Alternative Names: Nkx2-2; Nkx-2.2; Nkx2b; Homeobox protein Nkx-2.2; Homeobox protein NK-2 homolog B
Assay Type: Sandwich
Sensitivity: 0.069 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 1.972 1.891
5.00 1.599 1.518
2.50 1.196 1.115
1.25 0.813 0.732
0.63 0.527 0.446
0.32 0.319 0.238
0.16 0.206 0.125
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NKX2-2 and the recovery rates were calculated by comparing the measured value to the expected amount of NKX2-2 in samples.
Matrix Recovery range Average
serum(n=5) 83-95% 89%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKX2-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-95% 84-99% 87-96% 89-98%
EDTA plasma(n=5) 96-105% 95-103% 95-103% 84-98%
Heparin plasma(n=5) 93-102% 80-93% 91-100% 95-103%
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