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ELK Biotechnology

ELK9212

Rat hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

Rat hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

$458.00 USD
$458.00 USD
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Rat hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

Overview

Product name: Rat hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit
Reactivity: Rat
Alternative Names: High Sensitivity Cardiac Troponin I
Assay Type: Sandwich
Sensitivity: 4.94 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.266 2.164
500.00 1.715 1.613
250.00 1.226 1.124
125.00 0.933 0.831
62.50 0.527 0.425
31.25 0.364 0.262
15.63 0.236 0.134
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.
Matrix Recovery range Average
serum(n=5) 87-95% 91%
EDTA plasma(n=5) 96-107% 102%
Heparin plasma(n=5) 94-107% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 88-101% 86-97% 87-102%
EDTA plasma(n=5) 92-102% 81-93% 86-98% 84-98%
Heparin plasma(n=5) 89-105% 90-101% 87-96% 92-105%
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