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ELK Biotechnology

ELK9197

Human Pro-ADM(Proadrenomedullin) ELISA Kit

Human Pro-ADM(Proadrenomedullin) ELISA Kit

$458.00 USD
$458.00 USD
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Human Pro-ADM(Proadrenomedullin) ELISA Kit

Overview

Product name: Human Pro-ADM(Proadrenomedullin) ELISA Kit
Reactivity: Human
Alternative Names: Proadrenomedullin
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Pro-ADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Pro-ADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Pro-ADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Pro-ADM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.204 2.117
10.00 1.628 1.541
5.00 1.182 1.095
2.50 0.835 0.748
1.25 0.473 0.386
0.63 0.305 0.218
0.32 0.215 0.128
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Pro-ADM and the recovery rates were calculated by comparing the measured value to the expected amount of Pro-ADM in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 81-97% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pro-ADM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-96% 82-95% 86-99% 89-96%
EDTA plasma(n=5) 93-107% 93-102% 79-94% 87-98%
Heparin plasma(n=5) 86-97% 90-101% 92-102% 81-93%
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