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ELK Biotechnology

ELK9133

Human MHCF(Major Histocompatibility Complex Class I F) ELISA Kit

Human MHCF(Major Histocompatibility Complex Class I F) ELISA Kit

$458.00 USD
$458.00 USD
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Human MHCF(Major Histocompatibility Complex Class I F) ELISA Kit

Overview

Product name: Human MHCF(Major Histocompatibility Complex Class I F) ELISA Kit
Reactivity: Human
Alternative Names: CDA12; HLA 5.4; HLA CDA12; HLA F; HLA F antigen; HLAF; HLA-F; Leukocyte antigen F; MHC class I antigen F
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCF in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.268 2.180
500.00 1.663 1.575
250.00 1.134 1.046
125.00 0.872 0.784
62.50 0.572 0.484
31.25 0.379 0.291
15.63 0.179 0.091
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MHCF and the recovery rates were calculated by comparing the measured value to the expected amount of MHCF in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 85-97% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MHCF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 87-96% 78-92% 85-97%
EDTA plasma(n=5) 89-102% 92-101% 88-102% 89-103%
Heparin plasma(n=5) 82-95% 86-99% 87-95% 82-94%
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