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ELK Biotechnology

ELK9112

Human hs-Hepc25(high sensitivity Hepcidin 25) ELISA Kit

Human hs-Hepc25(high sensitivity Hepcidin 25) ELISA Kit

$458.00 USD
$458.00 USD
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Human hs-Hepc25(high sensitivity Hepcidin 25) ELISA Kit

Overview

Product name: Human hs-Hepc25(high sensitivity Hepcidin 25) ELISA Kit
Reactivity: Human
Alternative Names: Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; HEPCIDIN; Hepcidin 20; Hepcidin 25; Hepcidin antimicrobial peptide; Hepcidin-20; Hepcidin25; HFE2; HFE2B; LEAP 1; LEAP-1; LEAP1; Liver expressed antimicrobial peptide; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;
Assay Type: Sandwich
Sensitivity: 0.61 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular; Signal Transduction; Cardiovascular; Metabolism
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
100.00 1.867 1.773
50.00 1.429 1.335
25.00 1.052 0.958
12.50 0.897 0.803
6.25 0.475 0.381
3.13 0.385 0.291
1.57 0.215 0.121
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-Hepc25 and the recovery rates were calculated by comparing the measured value to the expected amount of hs-Hepc25 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 93-107% 100%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-Hepc25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-93% 95-106% 88-103% 81-98%
EDTA plasma(n=5) 78-90% 91-99% 97-103% 86-101%
Heparin plasma(n=5) 87-96% 92-105% 98-107% 93-102%
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