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ELK Biotechnology

ELK9102

Human EPCA2(Early Prostate Cancer Antigen 2) ELISA Kit

Human EPCA2(Early Prostate Cancer Antigen 2) ELISA Kit

$458.00 USD
$458.00 USD
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Human EPCA2(Early Prostate Cancer Antigen 2) ELISA Kit

Overview

Product name: Human MBP-C/MBL2(Mannose Binding Protein C/Mannose Binding Lectin) ELISA Kit
Reactivity: Human
Alternative Names: MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect); EPCA2; Early Prostate Cancer Antigen 2
Assay Type: Sandwich
Sensitivity: 2.45 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Signal Transduction; Cancer
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBP-C/MBL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBP-C/MBL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBP-C/MBL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBP-C/MBL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 1.932 1.845
200.00 1.515 1.428
100.00 1.067 0.980
50.00 0.892 0.805
25.00 0.553 0.466
12.50 0.389 0.302
6.25 0.161 0.074
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant MBP-C/MBL2 and the recovery rates were calculated by comparing the measured value to the expected amount of MBP-C/MBL2 in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 90-105% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBP-C/MBL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 85-92% 83-96% 93-102%
EDTA plasma(n=5) 86-94% 81-95% 98-105% 85-96%
Heparin plasma(n=5) 89-100% 85-96% 93-102% 87-96%
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