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ELK Biotechnology

ELK9080

Human hs-cTnI (High Sensitivity Cardiac Troponin I) ELISA Kit

Human hs-cTnI (High Sensitivity Cardiac Troponin I) ELISA Kit

$588.00 USD
$588.00 USD
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Human hs-cTnI (High Sensitivity Cardiac Troponin I) ELISA Kit

Overview

Product name: Human hs-cTnI (High Sensitivity Cardiac Troponin I) ELISA Kit
Reactivity: Human
Alternative Names: High Sensitivity Cardiac Troponin I
Assay Type: Sandwich
Sensitivity: 9.53 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.219 2.132
1000.00 1.703 1.616
500.00 1.174 1.087
250.00 0.847 0.760
125.00 0.559 0.472
62.50 0.361 0.274
31.25 0.216 0.129
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.
Matrix Recovery range Average
serum(n=5) 79-93% 86%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 88-102% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 92-101% 83-96% 93-105%
EDTA plasma(n=5) 79-86% 87-103% 85-97% 79-96%
Heparin plasma(n=5) 89-100% 85-96% 81-94% 93-107%
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