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ELK Biotechnology

ELK9033

Guinea pig NFkB(Nuclear Factor Kappa B) ELISA Kit

Guinea pig NFkB(Nuclear Factor Kappa B) ELISA Kit

$458.00 USD
$458.00 USD
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Guinea pig NFkB(Nuclear Factor Kappa B) ELISA Kit

Overview

Product name: Guinea pig NFkB(Nuclear Factor Kappa B) ELISA Kit
Reactivity: Guinea pig
Alternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B
Assay Type: Sandwich
Sensitivity: 0.114 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.952 1.865
10.00 1.582 1.495
5.00 1.282 1.195
2.50 0.852 0.765
1.25 0.576 0.489
0.63 0.324 0.237
0.32 0.248 0.161
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.
Matrix Recovery range Average
serum(n=5) 80-93% 86%
EDTA plasma(n=5) 81-95% 88%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-92% 86-94% 81-95% 88-97%
EDTA plasma(n=5) 85-92% 83-96% 93-102% 79-96%
Heparin plasma(n=5) 92-105% 91-99% 85-102% 79-92%
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