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ELK Biotechnology

ELK8994

Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

$376.00 USD
$376.00 USD
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Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Overview

Product name: Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity: Rat
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type: Competitive Inhibition
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Detection Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
6000.00 0.189 —
3000.00 0.332 —
1500.00 0.621 —
750.00 0.897 —
375.00 1.253 —
187.50 1.583 —
93.75 1.761 —
0.00 2.211 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 80-95% 97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 96-105% 88-106% 93-102%
EDTA plasma(n=5) 95-104% 97-106% 85-98% 81-92%
Heparin plasma(n=5) 95-106% 87-105% 92-101% 79-90%

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