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ELK Biotechnology

ELK8908

General 11-KT( 11-keto Testosterone) ELISA Kit

General 11-KT( 11-keto Testosterone) ELISA Kit

$376.00 USD
$376.00 USD
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General 11-KT( 11-keto Testosterone) ELISA Kit

Overview

Product name: 11-KT( 11-keto Testosterone) ELISA Kit
Reactivity: General
Alternative Names: 11-keto Testosterone
Assay Type: Competitive Inhibition
Sensitivity: 15.29 pg/mL
Standard: 10000 pg/mL
Detection Range: 156.25-10000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 11-KT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 11-KT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-KT in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10000.00 0.191 —
5000.00 0.397 —
2500.00 0.715 —
1250.00 0.823 —
625.00 1.257 —
312.50 1.572 —
156.25 1.769 —
0.00 2.232 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 11-KT and the recovery rates were calculated by comparing the measured value to the expected amount of 11-KT in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 80-95% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 11-KT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 79-94% 87-96% 87-101% 82-90%
EDTA plasma(n=5) 87-95% 78-91% 97-103% 95-101%
Heparin plasma(n=5) 85-93% 88-95% 96-102% 83-97%
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