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ELK Biotechnology

ELK8803

Cattle NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

Cattle NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Cattle NOS2(Nitric Oxide Synthase 2, Inducible) ELISA Kit

Overview

Product name: Cattle NOS2/iNOS(NitricOxide Synthase 2, Inducible) ELISA Kit
Reactivity: Cattle
Alternative Names: NOS2A; INOS; HEP-NOS; I-NOS; Hepatocytes Oxide Synthase; Peptidyl-cysteine S-nitrosylase NOS2; NOS2; Nitric Oxide Synthase 2, Inducible
Assay Type: Sandwich
Sensitivity: 0.126 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NOS2/iNOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NOS2/iNOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NOS2/iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NOS2/iNOS in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.941 1.873
10.00 1.647 1.579
5.00 1.144 1.076
2.50 0.883 0.815
1.25 0.516 0.448
0.63 0.389 0.321
0.32 0.236 0.168
0.00 0.068 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NOS2/iNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS2/iNOS in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 95-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS2/iNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 93-101% 85-98% 90-101%
EDTA plasma(n=5) 95-104% 93-105% 82-101% 91-103%
Heparin plasma(n=5) 87-98% 92-104% 82-96% 95-102%

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