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ELK Biotechnology

ELK8697

Goat CDHE(Cadherin, Epithelial) ELISA Kit

Goat CDHE(Cadherin, Epithelial) ELISA Kit

$376.00 USD
$376.00 USD
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Size
Sensitivity
Detection range

Goat CDHE(Cadherin, Epithelial) ELISA Kit

Overview

Product name: Goat E-Cad(E-Cadherin) ELISA Kit
Reactivity: Goat
Alternative Names: CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type: Sandwich
Sensitivity: 25.1 pg/mL
Standard: 4000 pg/mL
Range: 62.5-4000 pg/mL
Sample Type: serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
4000.00 2.126 2.024
2000.00 1.534 1.432
1000.00 1.182 1.080
500.00 0.896 0.794
250.00 0.527 0.425
125.00 0.385 0.283
62.50 0.157 0.055
0.00 0.102 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 86-99% 92%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 87-96% 96-105% 95-103%
EDTA plasma(n=5) 85-94% 87-101% 89-99% 85-95%
Heparin plasma(n=5) 95-104% 82-94% 86-96% 81-97%
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