1 1

ELK Biotechnology

ELK8625

Human HVD3(25-Hydroxyvitamin D3) ELISA Kit

Human HVD3(25-Hydroxyvitamin D3) ELISA Kit

$376.00 USD
$376.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Human HVD3(25-Hydroxyvitamin D3) ELISA Kit

Overview

Product name: Human HVD3(25-Hydroxyvitamin D3) ELISA Kit
Reactivity: Human
Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Assay Type: Competitive Inhibition
Sensitivity: 0.91 ng/mL
Standard: 200 ng/mL
Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 0.197 —
100.00 0.397 —
50.00 0.596 —
25.00 0.812 —
12.50 1.139 —
6.25 1.457 —
3.13 1.697 —
0.00 2.226 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant HVD3 and the recovery rates were calculated by comparing the measured value to the expected amount of HVD3 in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 90%
EDTA plasma(n=5) 80-97% 88%
Heparin plasma(n=5) 85-101% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HVD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 97-105% 87-102% 89-101%
EDTA plasma(n=5) 91-104% 82-96% 88-101% 90-99%
Heparin plasma(n=5) 85-104% 82-98% 89-97% 85-93%
View full details