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ELK Biotechnology

ELK8573

Human DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit

Human DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit

Overview

Product name: Human DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit
Reactivity: Human
Alternative Names: Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate
Assay Type: Competitive Inhibition
Sensitivity: 4.25 ng/mL
Standard: 400 ng/mL
Range: 6.25-400 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human DHEA-S protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 0.212 —
200.00 0.397 —
100.00 0.612 —
50.00 0.833 —
25.00 1.169 —
12.50 1.537 —
6.25 1.762 —
0.00 2.112 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant DHEA-S and the recovery rates were calculated by comparing the measured value to the expected amount of DHEA-S in samples.
Matrix Recovery range Average
serum(n=5) 88-96% 92%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA-S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 79-96% 85-101% 79-90%
EDTA plasma(n=5) 86-93% 93-101% 86-97% 90-101%
Heparin plasma(n=5) 85-94% 83-92% 89-98% 85-94%
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