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ELK Biotechnology

ELK8422

Rat Krebs Von den Lungen-6（KL-6)ELISA Kit

Rat Krebs Von den Lungen-6（KL-6)ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat Krebs Von den Lungen-6（KL-6)ELISA Kit

Overview

Product name: Rat Krebs Von den Lungen-6(KL-6) ELISA Kit
Reactivity: Rat
Alternative Names: KL-6
Assay Type: Competitive Inhibition
Sensitivity: 5.9 IU/mL
Standard: 800 IU/mL
Range: 12.5-800 IU/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Interstitial lung disease
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat KL-6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 0.197 —
400.00 0.336 —
200.00 0.611 —
100.00 0.927 —
50.00 1.119 —
25.00 1.511 —
12.50 1.755 —
0.00 2.211 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 83-95% 89%
Heparin plasma(n=5) 90-105% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 85-98% 85-94% 87-96%
EDTA plasma(n=5) 85-97% 87-96% 85-96% 85-95%
Heparin plasma(n=5) 85-94% 95-103% 79-97% 93-102%
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