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ELK Biotechnology

ELK8412

Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit

Overview

Product name: Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit
Reactivity: Human
Alternative Names: pMAPT /pTAU
Assay Type: Sandwich
Sensitivity: 12.37 pg/mL
Standard: 2000 pg/mL
Range: 31.25-2000 pg/mL
Sample Type: serum, plasma, other biological fluids
Assay Length: 3.5h
Research Area: intracellular signal transduction and neuron's development and viability.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT /pTAU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pMAPT /pTAU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pMAPT /pTAU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pMAPT /pTAU in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2000.00 2.195 2.109
1000.00 1.685 1.599
500.00 1.128 1.042
250.00 0.866 0.780
125.00 0.539 0.453
62.50 0.313 0.227
31.25 0.221 0.135
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant pMAPT /pTAU and the recovery rates were calculated by comparing the measured value to the expected amount of pMAPT /pTAU in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 85-99% 92%
Heparin plasma(n=5) 83-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pMAPT /pTAU and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 92-105% 89-102% 92-101%
EDTA plasma(n=5) 85-96% 86-95% 90-102% 81-90%
Heparin plasma(n=5) 87-95% 83-96% 92-102% 82-93%
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