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ELK Biotechnology

ELK8381

Human p-cresol ELISA Kit

Human p-cresol ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human p-cresol ELISA Kit

Overview

Product name: Human p-cresol ELISA Kit
Reactivity: Human
Alternative Names: 4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol
Assay Type: Sandwich
Sensitivity: 1.21 pg/mL
Standard: 200 pg/mL
Range: 3.13-200 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.131 2.048
100.00 1.647 1.564
50.00 1.174 1.091
25.00 0.728 0.645
12.50 0.518 0.435
6.25 0.262 0.179
3.13 0.175 0.092
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant p-cresol and the recovery rates were calculated by comparing the measured value to the expected amount of p-cresol in samples.
Matrix Recovery range Average
serum(n=5) 78-94% 86%
EDTA plasma(n=5) 90-103% 96%
Heparin plasma(n=5) 81-96% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-cresol and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-96% 86-95% 91-103% 87-93%
EDTA plasma(n=5) 97-105% 88-95% 96-102% 83-97%
Heparin plasma(n=5) 89-103% 85-93% 89-97% 95-102%
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