1 1

ELK Biotechnology

ELK8373

Rat 4-Hydroxynonenal (HNE)ELISA Kit

Rat 4-Hydroxynonenal (HNE)ELISA Kit

$458.00 USD
$458.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Rat 4-Hydroxynonenal (HNE)ELISA Kit

Overview

Product name: Rat 4-Hydroxynonenal(HNE) ELISA Kit
Reactivity: Rat
Alternative Names: 4-HNE; HNE; 4-hydroxy-2-nonenal; 4-Hydroxynonenal
Assay Type: Competitive Inhibition
Sensitivity: 7.54 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 4-HNE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.182 —
500.00 0.275 —
250.00 0.557 —
125.00 0.897 —
62.50 1.253 —
31.25 1.587 —
15.63 1.863 —
0.00 2.475 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 87-99% 86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 95-104% 87-96% 85-97%
EDTA plasma(n=5) 86-98% 91-103% 85-97% 87-96%
Heparin plasma(n=5) 83-94% 83-96% 92-102% 82-93%
View full details