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ELK Biotechnology

ELK8239

General VMA(Vanillylmandelic Acid) ELISA Kit

General VMA(Vanillylmandelic Acid) ELISA Kit

$376.00 USD
$376.00 USD
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General VMA(Vanillylmandelic Acid) ELISA Kit

Overview

Product name: VMA(Vanillylmandelic Acid) ELISA Kit
Reactivity: General
Alternative Names: Vanillyl Mandelic Acid
Assay Type: Competitive Inhibition
Sensitivity: 43.7 ng/mL
Standard: 10000 ng/mL
Range: 156.25-10000 ng/mL
Sample Type: urine and biological agents
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10000.00 0.219 —
5000.00 0.332 —
2500.00 0.577 —
1250.00 0.895 —
625.00 1.139 —
312.50 1.565 —
156.25 1.733 —
0.00 2.236 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant VMA and the recovery rates were calculated by comparing the measured value to the expected amount of VMA in samples.
Matrix Recovery range Average
serum(n=5) 82-95% 88%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 95-107% 96%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VMA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-93% 96-102% 81-93% 89-97%
EDTA plasma(n=5) 85-94% 95-103% 79-97% 93-102%
Heparin plasma(n=5) 79-85% 82-96% 92-101% 82-90%
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