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ELK Biotechnology

ELK8207

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit

Overview

Product name: Rat LL-37(Antibacterial Protein LL-37) ELISA Kit
Reactivity: Rat
Alternative Names: CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic; CAMP; Cathelicidin Antimicrobial Peptide
Assay Type: Sandwich
Sensitivity: 57 pg/mL
Standard: 8000 pg/mL
Range: 125-8000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
8000.00 2.052 1.965
4000.00 1.717 1.630
2000.00 1.282 1.195
1000.00 0.797 0.710
500.00 0.535 0.448
250.00 0.324 0.237
125.00 0.175 0.088
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LL-37 and the recovery rates were calculated by comparing the measured value to the expected amount of LL-37 in samples.
Matrix Recovery range Average
serum(n=5) 81-95% 88%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 83-98% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LL-37 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 79-92% 82-93% 83-97%
EDTA plasma(n=5) 78-92% 83-96% 95-107% 87-102%
Heparin plasma(n=5) 88-102% 86-97% 89-97% 79-95%
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