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ELK Biotechnology

ELK8182

Mouse BMX(BMX Non Receptor Tyrosine Kinase) ELISA Kit

Mouse BMX(BMX Non Receptor Tyrosine Kinase) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Mouse BMX(BMX Non Receptor Tyrosine Kinase) ELISA Kit

Overview

Product name: Mouse BMX(BMX Non Receptor Tyrosine Kinase) ELISA Kit
Reactivity: Mouse
Alternative Names: ETK; PSCTK3; NTK38; Epithelial and endothelial tyrosine kinase; Bone marrow tyrosine kinase gene in chromosome X; Cytoplasmic tyrosine-protein kinase BMX
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Range: 0.32-20 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BMX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BMX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BMX in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 1.996 1.908
10.00 1.685 1.597
5.00 1.107 1.019
2.50 0.782 0.694
1.25 0.532 0.444
0.63 0.319 0.231
0.32 0.235 0.147
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BMX and the recovery rates were calculated by comparing the measured value to the expected amount of BMX in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 87-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 93-106% 85-95% 92-104%
EDTA plasma(n=5) 86-97% 88-95% 92-101% 87-98%
Heparin plasma(n=5) 92-101% 82-98% 89-97% 85-93%
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