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ELK Biotechnology

ELK8046

Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit

Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit

Overview

Product name: Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit
Reactivity: Human
Alternative Names: anti-IgA
Assay Type: Sandwich
Sensitivity: 2.36 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;Pulmonology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 2.045 1.964
200.00 1.527 1.446
100.00 1.088 1.007
50.00 0.892 0.811
25.00 0.547 0.466
12.50 0.403 0.322
6.25 0.179 0.098
0.00 0.081 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Anti-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-IgA in samples.
Matrix Recovery range Average
serum(n=5) 83-97% 91%
EDTA plasma(n=5) 82-94% 88%
Heparin plasma(n=5) 94-105% 98%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-94% 87-101% 86-97% 88-95%
EDTA plasma(n=5) 96-103% 83-96% 87-98% 85-97%
Heparin plasma(n=5) 78-93% 96-102% 95-103% 89-97%
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