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ELK Biotechnology

ELK7861

General 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

General 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

$376.00 USD
$376.00 USD
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Size
Sensitivity
Detection range

General 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Overview

Product name: 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity: General
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type: Competitive Inhibition
Sensitivity: 26.29 pg/mL
Standard: 6000 pg/mL
Range: 93.75-6000 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
6000.00 0.221 —
3000.00 0.411 —
1500.00 0.692 —
750.00 0.913 —
375.00 1.225 —
187.50 1.587 —
93.75 1.813 —
0.00 2.325 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 94-105% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 92-101% 80-93% 89-97%
EDTA plasma(n=5) 85-90% 83-96% 95-102% 88-95%
Heparin plasma(n=5) 92-102% 83-89% 88-104% 97-103%
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