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ELK Biotechnology

ELK7687

Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Overview

Product name: Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Pig
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Competitive Inhibition
Sensitivity: 43.8 pg/mL
Standard: 10000 pg/mL
Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig NT-ProBNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10000.00 0.185 —
5000.00 0.397 —
2500.00 0.655 —
1250.00 0.856 —
625.00 1.139 —
312.50 1.578 —
156.25 1.799 —
0.00 2.101 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
Matrix Recovery range Average
serum(n=5) 79-93% 86%
EDTA plasma(n=5) 90-105% 97%
Heparin plasma(n=5) 86-99% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 86-94% 93-102% 85-98% 90-101%
EDTA plasma(n=5) 91-99% 83-97% 87-105% 87-99%
Heparin plasma(n=5) 89-97% 95-104% 82-96% 85-98%
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