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ELK Biotechnology

ELK6913

Mouse 20S-PSM(20S-Proteasome) ELISA Kit

Mouse 20S-PSM(20S-Proteasome) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Mouse 20S-PSM(20S-Proteasome) ELISA Kit

Overview

Product name: Mouse 20S-PSM(20S-Proteasome) ELISA Kit
Reactivity: Mouse
Alternative Names: CP; 20SPSM; 20S Core Particle
Assay Type: Competitive Inhibition
Sensitivity: 152.4 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 2h
Research Area: Enzyme & Kinase;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 20S-PSM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 20S-PSM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 20S-PSM in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
30000.00 0.191 —
15000.00 0.275 —
7500.00 0.527 —
3750.00 0.842 —
1875.00 1.169 —
937.50 1.585 —
468.75 1.624 —
0.00 2.256 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant 20S-PSM and the recovery rates were calculated by comparing the measured value to the expected amount of 20S-PSM in samples.
Matrix Recovery range Average
serum(n=5) 88-102% 95%
EDTA plasma(n=5) 79-97% 88%
Heparin plasma(n=5) 83-95% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 20S-PSM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 80-96% 87-105% 90-99%
EDTA plasma(n=5) 88-102% 86-92% 81-93% 79-87%
Heparin plasma(n=5) 88-102% 86-92% 83-98% 96-103%
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