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ELK Biotechnology

ELK6488

Cattle PPARg(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Cattle PPARg(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Cattle PPARg(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit

Overview

Product name: Cattle PPAR-γ(Peroxisome Proliferator Activated Receptor Gamma) ELISA Kit
Reactivity: Cattle
Alternative Names: PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3; PPARg; Peroxisome Proliferator Activated Receptor Gamma
Assay Type: Sandwich
Sensitivity: 0.133 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Developmental science;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PPAR-γ. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PPAR-γ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle PPAR-γ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle PPAR-γ in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.126 2.030
10.00 1.587 1.491
5.00 1.043 0.947
2.50 0.815 0.719
1.25 0.529 0.433
0.63 0.315 0.219
0.32 0.249 0.153
0.00 0.096 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant PPAR-γ and the recovery rates were calculated by comparing the measured value to the expected amount of PPAR-γ in samples.
Matrix Recovery range Average
serum(n=5) 95-108% 101%
EDTA plasma(n=5) 83-97% 90%
Heparin plasma(n=5) 80-97% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPAR-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 86-95% 90-102% 87-98%
EDTA plasma(n=5) 87-96% 82-95% 93-102% 87-96%
Heparin plasma(n=5) 85-94% 83-96% 87-98% 87-100%
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