1 1

ELK Biotechnology

ELK6409

Pig PTH(Parathyroid Hormone) ELISA Kit

Pig PTH(Parathyroid Hormone) ELISA Kit

$588.00 USD
$588.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Pig PTH(Parathyroid Hormone) ELISA Kit

Overview

Product name: Pig I-PTH(IntactParathormone) ELISA Kit
Reactivity: Pig
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type: Sandwich
Sensitivity: 5.33 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 1.948 1.860
500.00 1.643 1.555
250.00 1.139 1.051
125.00 0.897 0.809
62.50 0.538 0.450
31.25 0.335 0.247
15.63 0.175 0.087
0.00 0.088 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix Recovery range Average
serum(n=5) 81-93% 87%
EDTA plasma(n=5) 78-90% 85%
Heparin plasma(n=5) 93-106% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 78-92% 83-96% 87-98% 85-92%
EDTA plasma(n=5) 78-92% 79-93% 91-98% 89-102%
Heparin plasma(n=5) 87-98% 91-105% 87-98% 89-94%
View full details