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ELK Biotechnology

ELK6044

Dog PTH(Parathyroid Hormone) ELISA Kit

Dog PTH(Parathyroid Hormone) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Dog PTH(Parathyroid Hormone) ELISA Kit

Overview

Product name: Dog I-PTH(IntactParathormone) ELISA Kit
Reactivity: Dog
Alternative Names: iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH; Parathyroid Hormone
Assay Type: Sandwich
Sensitivity: 4.15 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog I-PTH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog I-PTH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog I-PTH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog I-PTH in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 1.967 1.881
400.00 1.647 1.561
200.00 1.183 1.097
100.00 0.907 0.821
50.00 0.539 0.453
25.00 0.356 0.270
12.50 0.189 0.103
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant I-PTH and the recovery rates were calculated by comparing the measured value to the expected amount of I-PTH in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 88-102% 95%
Heparin plasma(n=5) 90-105% 100%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of I-PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 82-96% 86-97% 90-101%
EDTA plasma(n=5) 91-99% 85-93% 82-95% 93-99%
Heparin plasma(n=5) 82-94% 92-104% 82-96% 95-102%
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