ELK Biotechnology
SKU:ELK5775
Rat GDF15(Growth Differentiation Factor 15) ELISA Kit
Rat GDF15(Growth Differentiation Factor 15) ELISA Kit
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$458.00 USD
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$458.00 USD
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Introducing the Rat GDF15 (Growth Differentiation Factor 15) ELISA Kit, a cutting-edge solution designed to accurately measure and quantify GDF15 levels in rat samples. This high-quality kit is meticulously crafted to provide researchers and scientists with reliable and precise results, enabling them to delve deeper into the intricate mechanisms of GDF15.
The Rat GDF15 ELISA Kit boasts a comprehensive and user-friendly design, ensuring ease of use and efficiency in laboratory settings. With its advanced technology and optimized protocols, this kit guarantees exceptional sensitivity and specificity, allowing for the detection of even the lowest concentrations of GDF15 in rat samples.
This ELISA kit is meticulously manufactured using top-grade materials and adheres to stringent quality control measures, ensuring consistent and reproducible results. Its robust and reliable performance makes it an indispensable tool for a wide range of applications, including biomedical research, drug development, and clinical diagnostics.
The Rat GDF15 ELISA Kit comes complete with all necessary components, including pre-coated microplates, standards, detection antibodies, and reagents, simplifying the experimental process and minimizing the risk of errors. The kit also includes a comprehensive instruction manual, providing step-by-step guidance for seamless execution of the assay.
With its exceptional accuracy, precision, and ease of use, the Rat GDF15 ELISA Kit is an invaluable asset for researchers and scientists seeking to unravel the complexities of GDF15 in rat samples. Trust in this state-of-the-art kit to deliver reliable and reproducible results, empowering you to advance your research and contribute to the scientific community's understanding of GDF15 biology.
The Rat GDF15 ELISA Kit boasts a comprehensive and user-friendly design, ensuring ease of use and efficiency in laboratory settings. With its advanced technology and optimized protocols, this kit guarantees exceptional sensitivity and specificity, allowing for the detection of even the lowest concentrations of GDF15 in rat samples.
This ELISA kit is meticulously manufactured using top-grade materials and adheres to stringent quality control measures, ensuring consistent and reproducible results. Its robust and reliable performance makes it an indispensable tool for a wide range of applications, including biomedical research, drug development, and clinical diagnostics.
The Rat GDF15 ELISA Kit comes complete with all necessary components, including pre-coated microplates, standards, detection antibodies, and reagents, simplifying the experimental process and minimizing the risk of errors. The kit also includes a comprehensive instruction manual, providing step-by-step guidance for seamless execution of the assay.
With its exceptional accuracy, precision, and ease of use, the Rat GDF15 ELISA Kit is an invaluable asset for researchers and scientists seeking to unravel the complexities of GDF15 in rat samples. Trust in this state-of-the-art kit to deliver reliable and reproducible results, empowering you to advance your research and contribute to the scientific community's understanding of GDF15 biology.
Overview
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.266 | 2.180 |
| 500.00 | 1.643 | 1.557 |
| 250.00 | 1.169 | 1.083 |
| 125.00 | 0.826 | 0.740 |
| 62.50 | 0.487 | 0.401 |
| 31.25 | 0.345 | 0.259 |
| 15.63 | 0.211 | 0.125 |
| 0.00 | 0.086 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant GDF15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF15 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 87-99% | 93% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 95-107% | 101% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-102% | 86-92% | 91-103% | 89-97% |
| EDTA plasma(n=5) | 95-107% | 92-103% | 81-99% | 90-98% |
| Heparin plasma(n=5) | 85-92% | 83-96% | 93-102% | 87-98% |
