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ELK Biotechnology

ELK5712

Guinea pig EDN1(Endothelin 1) ELISA Kit

Guinea pig EDN1(Endothelin 1) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Guinea pig EDN1(Endothelin 1) ELISA Kit

Overview

Product name: Guinea pig ET-1(Endothelin1) ELISA Kit
Reactivity: Guinea pig
Alternative Names: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Assay Type: Sandwich
Sensitivity: 2.82 pg/mL
Standard: 500 pg/mL
Range: 7.82-500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
500.00 2.126 2.025
250.00 1.528 1.427
125.00 1.147 1.046
62.50 0.806 0.705
31.25 0.577 0.476
15.63 0.315 0.214
7.82 0.215 0.114
0.00 0.101 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ET-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ET-1 in samples.
Matrix Recovery range Average
serum(n=5) 86-99% 92%
EDTA plasma(n=5) 89-105% 97%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ET-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-92% 96-102% 81-93% 79-91%
EDTA plasma(n=5) 86-93% 93-101% 86-97% 90-101%
Heparin plasma(n=5) 91-99% 83-89% 88-104% 97-103%
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