1 1

ELK Biotechnology

ELK5694

Rat IL2Ra(Interleukin 2 Receptor Alpha) ELISA Kit

Rat IL2Ra(Interleukin 2 Receptor Alpha) ELISA Kit

$458.00 USD
$458.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Rat IL2Ra(Interleukin 2 Receptor Alpha) ELISA Kit

Overview

Product name: Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Reactivity: Rat
Alternative Names: IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type: Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.345 2.252
5.00 1.711 1.618
2.50 1.226 1.133
1.25 0.933 0.840
0.63 0.516 0.423
0.32 0.364 0.271
0.16 0.206 0.113
0.00 0.093 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant IL-2sRa/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2sRa/CD25 in samples.
Matrix Recovery range Average
serum(n=5) 85-97% 91%
EDTA plasma(n=5) 79-93% 86%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2sRa/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-94% 86-96% 81-97%
EDTA plasma(n=5) 86-93% 93-101% 86-97% 90-101%
Heparin plasma(n=5) 91-99% 88-97% 88-104% 97-108%
View full details