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ELK Biotechnology

ELK5585

Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit

Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit

Overview

Product name: Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
Reactivity: Human
Alternative Names: Proneurotensin 1-117
Assay Type: Competitive Inhibition
Sensitivity: 5.52 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Neuro science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ProNT/NMN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ProNT/NMN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ProNT/NMN in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 0.175 —
500.00 0.367 —
250.00 0.611 —
125.00 0.833 —
62.50 1.156 —
31.25 1.511 —
15.63 1.762 —
0.00 2.123 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ProNT/NMN and the recovery rates were calculated by comparing the measured value to the expected amount of ProNT/NMN in samples.
Matrix Recovery range Average
serum(n=5) 77-95% 86%
EDTA plasma(n=5) 88-103% 95%
Heparin plasma(n=5) 92-106% 95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ProNT/NMN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 89-103% 87-98% 95-102%
EDTA plasma(n=5) 98-105% 86-92% 87-93% 95-103%
Heparin plasma(n=5) 87-102% 92-105% 83-92% 89-98%
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