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ELK Biotechnology

ELK5540

Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

$588.00 USD
$588.00 USD
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Size
Sensitivity
Detection range

Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Overview

Product name: Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Rabbit
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Competitive Inhibition
Sensitivity: 47.2 pg/mL
Standard: 10000 pg/mL
Range: 156.25-10000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit NT-ProBNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10000.00 0.197 —
5000.00 0.310 —
2500.00 0.446 —
1250.00 0.676 —
625.00 0.763 —
312.50 1.439 —
156.25 1.624 —
0.00 2.236 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 87-99% 93%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 89-101% 87-98% 85-93%
EDTA plasma(n=5) 91-103% 86-92% 88-97% 89-97%
Heparin plasma(n=5) 95-107% 88-103% 81-98% 93-102%
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