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ELK Biotechnology

ELK5423

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Overview

Product name: Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Human
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Competitive Inhibition
Sensitivity: 12.2 pg/mL
Standard: 2500 pg/mL
Range: 39.07-2500 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NT-ProBNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
2500.00 0.235 —
1250.00 0.331 —
625.00 0.615 —
312.50 0.873 —
156.25 1.139 —
78.13 1.557 —
39.07 1.799 —
0.00 2.101 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 92-107% 101%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 90-99% 90-99% 82-101%
EDTA plasma(n=5) 92-105% 85-101% 85-94% 87-96%
Heparin plasma(n=5) 82-96% 85-97% 85-94% 87-96%
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