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ELK Biotechnology

ELK5390

Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit

Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit

$458.00 USD
$458.00 USD
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Size
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Detection range

Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit

Overview

Product name: Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit
Reactivity: Human
Alternative Names: BAPX2; NKX3; NKX3.1; NKX3A; NK3 transcription factor related, locus 1; Homeobox protein NK-3 homolog A
Assay Type: Sandwich
Sensitivity: 0.055 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX3-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX3-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX3-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX3-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
10.00 2.095 2.001
5.00 1.697 1.603
2.50 1.124 1.030
1.25 0.661 0.567
0.63 0.593 0.499
0.32 0.327 0.233
0.16 0.225 0.131
0.00 0.094 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NKX3-1 and the recovery rates were calculated by comparing the measured value to the expected amount of NKX3-1 in samples.
Matrix Recovery range Average
serum(n=5) 84-102% 93%
EDTA plasma(n=5) 87-95% 90%
Heparin plasma(n=5) 80-95% 87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKX3-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 87-96% 82-93% 89-97%
EDTA plasma(n=5) 86-95% 93-103% 87-98% 85-96%
Heparin plasma(n=5) 91-99% 88-97% 88-104% 97-108%
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