1 1

ELK Biotechnology

ELK5354

Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit

Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit

$458.00 USD
$458.00 USD
Sale Sold out
Shipping calculated at checkout.
Size
Sensitivity
Detection range

Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit

Overview

Product name: Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit
Reactivity: Human
Alternative Names: DHC1b; DHC2; DNCH2; DYH1B; hdhc11; Dynein heavy chain isotype 1B; Dynein heavy chain 11; Dynein cytoplasmic heavy chain 2; Cytoplasmic dynein 2 heavy chain
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DYNC2H1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DYNC2H1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DYNC2H1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DYNC2H1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
20.00 2.179 2.071
10.00 1.603 1.495
5.00 1.157 1.049
2.50 0.705 0.597
1.25 0.515 0.407
0.63 0.352 0.244
0.32 0.158 0.050
0.00 0.108 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant DYNC2H1 and the recovery rates were calculated by comparing the measured value to the expected amount of DYNC2H1 in samples.
Matrix Recovery range Average
serum(n=5) 78-92% 85%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 83-97% 90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DYNC2H1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 93-101% 97-103% 91-99%
EDTA plasma(n=5) 85-92% 79-96% 89-101% 85-97%
Heparin plasma(n=5) 86-100% 93-108% 80-101% 87-96%
View full details