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ELK Biotechnology

ELK5326

Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit

Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit

$458.00 USD
$458.00 USD
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Size
Sensitivity
Detection range

Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit

Overview

Product name: Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit
Reactivity: Human
Alternative Names: CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
Assay Type: Sandwich
Sensitivity: 33 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 2.266 2.184
2500.00 1.790 1.708
1250.00 1.271 1.189
625.00 0.847 0.765
312.50 0.476 0.394
156.25 0.298 0.216
78.13 0.226 0.144
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant LAB7-1 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-1 in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 87-95% 91%
Heparin plasma(n=5) 78-90% 84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAB7-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 83-94% 85-98% 85-97%
EDTA plasma(n=5) 92-101% 87-98% 85-92% 79-96%
Heparin plasma(n=5) 95-103% 86-92% 89-97% 91-103%
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